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ProductsBack/CCNB1 Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human lung Tissue using Cyclin B1 Mouse mAb diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Human lung Tissue using Cyclin B1 Mouse mAb diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Human lung Tissue using Cyclin B1 Mouse mAb diluted at 1:200.

CCNB1 Monoclonal Antibody

CSB-MA124445
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCNB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCNB1 Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID891
  • Target name
    CCNB1
  • Target description
    cyclin B1
  • Target synonyms
    CCNB; G2/mitotic-specific cyclin-B1
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDP14635
  • Protein Name
    G2/mitotic-specific cyclin-B1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # A00745-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.
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