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ProductsBack/Cyclin B1 Polyclonal Antibody
HeLa lysates probed with Anti –(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.
HeLa lysates probed with Anti –(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.
HeLa lysates probed with Anti –(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.

Cyclin B1 Polyclonal Antibody

BS-0572R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCCNB1
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Overview

  • Supplier
    Bioss
  • Product Name
    Cyclin B1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID891
  • Target name
    CCNB1
  • Target description
    cyclin B1
  • Target synonyms
    CCNB, G2/mitotic-specific cyclin-B1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14635
  • Protein Name
    G2/mitotic-specific cyclin-B1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Maziero RRD, Guaitolini CRF, Paschoal DM, et al. Effects of the addition of oocyte meiosis-inhibiting drugs on the expression of maturation-promoting factor components and organization of cytoplasmic organelles. Reprod Biol. 2020,20(1):48-62. doi: 10.1016/j.repbio.2019.12.005
    Read this paper
  • Zhang L, Wei J, Duan J, et al. Silica nanoparticles exacerbates reproductive toxicity development in high-fat diet-treated Wistar rats. J Hazard Mater. 2020,384:121361. doi: 10.1016/j.jhazmat.2019.121361
    Read this paper
  • Zeng Q, Yi H, Huang L, et al. Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice. Toxicology. 2019,411:122-132. doi: 10.1016/j.tox.2018.09.010
    Read this paper
  • Wang Q, Wei LW, Xiao HQ, et al. Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies. Food Chem Toxicol. 2017,105:61-72. doi: 10.1016/j.fct.2017.03.030
    Read this paper
  • Ghate NB, Das A, Chaudhuri D, et al. Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio. Cell Death Discov. 2016,2:15062. doi: 10.1038/cddiscovery.2015.62
    Read this paper
  • Qu Z, Zou X, Zhang X, et al. Chelidonine induces mitotic slippage and apoptotic-like death in SGC-7901 human gastric carcinoma cells. Mol Med Rep. 2016,13(2):1336-44. doi: 10.3892/mmr.2015.4683
    Read this paper
  • Guo H, Cui H, Peng X, et al. Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney. Oncotarget. 2015,6(34):35964-77. doi: 10.18632/oncotarget.5934
    Read this paper

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Figure 1. Western blot analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # A00745-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.
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