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ProductsBack/Cyclin B1 Polyclonal Antibody
HeLa lysates probed with Anti –(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.
HeLa lysates probed with Anti –(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.
HeLa lysates probed with Anti –(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.

Cyclin B1 Polyclonal Antibody

BS-0572R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCCNB1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CCNB1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID891
  • Target name
    CCNB1
  • Target description
    cyclin B1
  • Target synonyms
    CCNB; G2/mitotic-specific cyclin-B1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14635
  • Protein Name
    G2/mitotic-specific cyclin-B1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Effects of the addition of oocyte meiosis-inhibiting drugs on the expression of maturation-promoting factor components and organization of cytoplasmic organelles. Maziero RRD et al., 2020 Mar, Reprod Biol
    Read more
  • Silica nanoparticles exacerbates reproductive toxicity development in high-fat diet-treated Wistar rats. Zhang L et al., 2020 Feb 15, J Hazard Mater
    Read more
  • Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice. Zeng Q et al., 2019 Jan 1, Toxicology
    Read more
  • Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies. Wang Q et al., 2017 Jul, Food Chem Toxicol
    Read more
  • Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio. Ghate NB et al., 2016, Cell Death Discov
    Read more
  • Chelidonine induces mitotic slippage and apoptotic-like death in SGC-7901 human gastric carcinoma cells. Qu Z et al., 2016 Feb, Mol Med Rep
    Read more
  • Dietary NiCl2 causes G2/M cell cycle arrest in the broilers kidney. Guo H et al., 2015 Nov 3, Oncotarget
    Read more

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Figure 1. Western blot analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # A00745-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.
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