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Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA004832LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA004832LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA004832LA01HU at dilution of 1:100

CCNT1 Antibody

CSB-PA004832LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCCNT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCNT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID904
  • Target name
    CCNT1
  • Target description
    cyclin T1
  • Target synonyms
    CCNT; CDK9-associated C-type protein; cyclin C-related protein; cyclin-T1; CYCT1; HIVE1; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1; MLLT10/CCNT1 fusion
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60563
  • Protein Name
    Cyclin-T1
  • Scientific Description
    Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cyclin T1 using anti-Cyclin T1 antibody (PB9990). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin T1 antigen affinity purified polyclonal antibody (Catalog # PB9990) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin T1 at approximately 81 kDa. The expected band size for Cyclin T1 is at 81 kDa.
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