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ProductsBack/CCT2 / CCT Beta Antibody

CCT2 / CCT Beta Antibody

LS-C335687
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCCT2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CCT2 / CCT Beta Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 52kDa/57kDa, while the observed MW by Western blot was 57kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 52kDa/57kDa, while the observed MW by Western blot was 57kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10576
  • Target name
    CCT2
  • Target description
    chaperonin containing TCP1 subunit 2
  • Target synonyms
    99D8.1; CCTB; CCT-beta; chaperonin containing t-complex polypeptide 1, beta subunit; chaperonin containing t-complex polypeptide 1, subunit 2; chaperonin containing TCP1, subunit 2 (beta); epididymis secretory sperm binding protein Li 100n; HEL-S-100n; PRO1633; T-complex protein 1 subunit beta; T-complex protein 1, beta subunit; TCP-1-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCT2 using anti-CCT2 antibody (PB9992). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human HEL whole cell lysates, Lane 7: human Raji whole cell lysates, Lane 8: human SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT2 antigen affinity purified polyclonal antibody (Catalog # PB9992) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT2 at approximately 57 kDa. The expected band size for CCT2 is at 57 kDa.
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References
GeneTex
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