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ProductsBack/TCP1 beta Recombinant Antibody, AbBy Fluor-555 Conjugated

TCP1 beta Recombinant Antibody, AbBy Fluor-555 Conjugated

BSM-62080R-BF555
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCCT2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    TCP1 beta Recombinant Antibody, AbBy Fluor-555 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID10576
  • Target name
    CCT2
  • Target description
    chaperonin containing TCP1 subunit 2
  • Target synonyms
    99D8.1; CCTB; CCT-beta; chaperonin containing t-complex polypeptide 1, beta subunit; chaperonin containing t-complex polypeptide 1, subunit 2; chaperonin containing TCP1, subunit 2 (beta); epididymis secretory sperm binding protein Li 100n; HEL-S-100n; PRO1633; T-complex protein 1 subunit beta; T-complex protein 1, beta subunit; TCP-1-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78371
  • Protein Name
    T-complex protein 1 subunit beta
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCT2 using anti-CCT2 antibody (PB9992). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human HEL whole cell lysates, Lane 7: human Raji whole cell lysates, Lane 8: human SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT2 antigen affinity purified polyclonal antibody (Catalog # PB9992) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT2 at approximately 57 kDa. The expected band size for CCT2 is at 57 kDa.
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Western blot All lanes: CCT2 antibody at 5.63ug/ml Lane 1: Jurkat whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 53 kDa Observed band size: 58 kDa
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