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Western blot All lanes: T-complex protein 1 subunit gamma antibody at 12ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 61, 57 kDa Observed band size: 61 kDa
Western blot All lanes: T-complex protein 1 subunit gamma antibody at 12ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 61, 57 kDa Observed band size: 61 kDa
Western blot All lanes: T-complex protein 1 subunit gamma antibody at 12ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 61, 57 kDa Observed band size: 61 kDa

CCT3 Antibody

CSB-PA004857ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCCT3
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCT3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7203
  • Target name
    CCT3
  • Target description
    chaperonin containing TCP1 subunit 3
  • Target synonyms
    CCT-gamma, CCTG, NEDSVH, PIG48, TCP-1-gamma, TRIC5, T-complex protein 1 subunit gamma, T-complex protein 1, gamma subunit, TCP1 (t-complex-1) ring complex, polypeptide 5, chaperonin containing T-complex polypeptide 1 subunit 3, chaperonin containing TCP1, subunit 3 (gamma), hTRiC5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49368
  • Protein Name
    T-complex protein 1 subunit gamma
  • Scientific Description
    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-CCT3 antibody (PB9926). Overlay histogram showing PC-3 cells stained with PB9926 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT3 Antibody (PB9926,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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