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Western blot analysis of extracts from HepG2 cells, using CCT5 antibody.
Western blot analysis of extracts from HepG2 cells, using CCT5 antibody.
Western blot analysis of extracts from HepG2 cells, using CCT5 antibody.

CCT5 Antibody

CSB-PA111204
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCCT5
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCT5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22948
  • Target name
    CCT5
  • Target description
    chaperonin containing TCP1 subunit 5
  • Target synonyms
    CCT-epsilon, CCTE, HEL-S-69, HSNSP, PNAS-102, TCP-1-epsilon, T-complex protein 1 subunit epsilon, chaperonin containing T-complex polypeptide 1 subunit 5, chaperonin containing TCP1, subunit 5 (epsilon), epididymis secretory protein Li 69
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48643
  • Protein Name
    T-complex protein 1 subunit epsilon
  • Scientific Description
    Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Sugano S., Nat. Genet. 36:40-45(2004). Venter J.C., Submitted (SEP-2005). Lukashchuk N., Submitted (MAR-2008).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes: CCT5 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 50 kDa Observed band size: 60 kDa
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Figure 1. Western blot analysis of CCT5 using anti-CCT5 antibody (PB9928). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human U20S whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse testis tissue lysates, Lane 11: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT5 antigen affinity purified polyclonal antibody (Catalog # PB9928) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT5 at approximately 60 kDa. The expected band size for CCT5 is at 60 kDa.
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