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CCT7 Antibody (aa398-447)

LS-C81666
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCCT7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CCT7 Antibody (aa398-447)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10574
  • Target name
    CCT7
  • Target description
    chaperonin containing TCP1 subunit 7
  • Target synonyms
    CCTETA; CCT-eta; CCTH; chaperonin containing t-complex polypeptide 1, eta subunit; HIV-1 Nef interacting protein; NIP7-1; T-complex protein 1 subunit eta; TCP1ETA; TCP-1-eta
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CCT7 antibody (A08169-2). Overlay histogram showing HepG2 cells stained with A08169-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT7 Antibody (A08169-2,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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