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Paraformaldehyde-fixed, paraffin-embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (bs-0579R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (bs-0579R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin-embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD105 Polyclonal Antibody, Unconjugated (bs-0579R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

CD105 Polyclonal Antibody

BS-0579R
Bioss
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetENG
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Overview

  • Supplier
    Bioss
  • Product Name
    CD105 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2022
  • Target name
    ENG
  • Target description
    endoglin
  • Target synonyms
    END, HHT1, ORW1, endoglin, CD105 antigen, soluble endoglin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17813
  • Protein Name
    Endoglin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

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  • Chen F, Zhao C, Zhao Y, et al. The biological characteristics of sheep umbilical cord mesenchymal stem cells. Can J Vet Res. 2018,82(3):216-224.
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  • Hussien NI, Ebrahim N, Mohammed OM, et al. Combination of Obestatin and Bone Marrow Mesenchymal Stem Cells Prevents Aggravation of Endocrine Pancreatic Damage in Type II Diabetic Rats. Int J Stem Cells. 2017,10(2):129-143. doi: 10.15283/ijsc17035
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  • Li M, Cai H, Yang Y, et al. Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway. Oncol Rep. 2016,36(2):936-44. doi: 10.3892/or.2016.4853
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  • Naderi-Meshkin H, Matin MM, Heirani-Tabasi A, et al. Injectable hydrogel delivery plus preconditioning of mesenchymal stem cells: exploitation of SDF-1/CXCR4 axis toward enhancing the efficacy of stem cells' homing. Cell Biol Int. 2016,40(7):730-41. doi: 10.1002/cbin.10474
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  • Boopathy AV, Pendergrass KD, Che PL, et al. Oxidative stress-induced Notch1 signaling promotes cardiogenic gene expression in mesenchymal stem cells. Stem Cell Res Ther. 2013,4(2):43. doi: 10.1186/scrt190
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  • Gao LR, Zhang NK, Ding QA, et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. Cell Transplant. 2013,22(10):1883-900. doi: 10.3727/096368912X662444
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