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Immunohistochemistry analysis of paraffin-embedded human lung cancer using CD107b Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using CD107b Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using CD107b Polyclonal Antibody at dilution of 1:300.

CD107b Polyclonal Antibody

E-AB-70198
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CD107b Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Lysosomal-associated membrane protein 2 (LAMP2,synonyms: LAMPB,CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection,maintenance,and adhesion of the lysosome. Prior to posttranslational modification,LAMP2 is a ~45 kDa polypeptide. Mature,functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LAMP2 using anti-LAMP2 antibody (A01573-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP2 antigen affinity purified polyclonal antibody (Catalog # A01573-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMP2 at approximately 100-110 kDa. The expected band size for LAMP2 is at 45 kDa.
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