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Human U937 cells probed with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human U937 cells probed with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human U937 cells probed with LAMP2 Polyclonal Antibody, Unconjugated (bs-2379R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

LAMP2 Polyclonal Antibody

BS-2379R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetLAMP2
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Overview

  • Supplier
    Bioss
  • Product Name
    LAMP2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3920
  • Target name
    LAMP2
  • Target description
    lysosomal associated membrane protein 2
  • Target synonyms
    CD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110, lysosome-associated membrane glycoprotein 2, CD107 antigen-like family member B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13473
  • Protein Name
    Lysosome-associated membrane glycoprotein 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Dong W, Tan Y, Qin Q, et al. Polybrominated Diphenyl Ethers Quinone Induces NCOA4-Mediated Ferritinophagy through Selectively Autophagic Degradation of Ferritin. Chem Res Toxicol. 2019,32(12):2509-2516. doi: 10.1021/acs.chemrestox.9b00350
    Read this paper
  • Iwao Y, Ojima H, Kobayashi T, et al. Liver atrophy after percutaneous transhepatic portal embolization occurs in two histological phases: Hepatocellular atrophy followed by apoptosis. World J Hepatol. 2017,9(32):1227-1238. doi: 10.4254/wjh.v9.i32.1227
    Read this paper
  • Kameyama H, Nakajima H, Nishitsuji K, et al. Iowa Mutant Apolipoprotein A-I (ApoA-IIowa) Fibrils Target Lysosomes. Sci Rep. 2016,6:30391. doi: 10.1038/srep30391
    Read this paper

Datasheet

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Figure 1. Western blot analysis of LAMP2 using anti-LAMP2 antibody (A01573-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP2 antigen affinity purified polyclonal antibody (Catalog # A01573-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMP2 at approximately 100-110 kDa. The expected band size for LAMP2 is at 45 kDa.
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