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Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

CD146 antibody [C1C3]

GTX108777
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMCAM
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD146 antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.21 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4162
  • Target name
    MCAM
  • Target description
    melanoma cell adhesion molecule
  • Target synonyms
    CD146, HEMCAM, METCAM, MUC18, MelCAM, cell surface glycoprotein MUC18, Gicerin, S-endo 1 endothelial-associated antigen, cell surface glycoprotein P1H12, melanoma adhesion molecule, melanoma-associated antigen A32, melanoma-associated antigen MUC18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43121
  • Protein Name
    Cell surface glycoprotein MUC18
  • Scientific Description
    Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Suardi D, Toriq H, Tuasikal RR, et al. Comparison of Extravillous Intermediate Trophoblast Invasion Depth and Distribution Pattern Between Placenta Accreta and Non-Accreta. Med Sci Monit. 2023,29:e939125. doi: 10.12659/MSM.939125
    Read this paper
  • Pearse DD, Otero PA, Diaz A, et al. Neuronal and Endothelial Transglutaminase-2 Expression during Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis. Neuroscience. 2021,461:140-154. doi: 10.1016/j.neuroscience.2020.11.034
    Read this paper
  • Hu Y, Wu H, Xu T, et al. Defactinib attenuates osteoarthritis by inhibiting positive feedback loop between H-type vessels and MSCs in subchondral bone. J Orthop Translat. 2020,24:12-22. doi: 10.1016/j.jot.2020.04.008
    Read this paper
  • Chen SY, Lin MC, Tsai JS, et al. Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain. Stem Cells Transl Med. 2020,9(4):499-517. doi: 10.1002/sctm.19-0174
    Read this paper
  • Akiyama Y, Shinose M, Watanabe H, et al. Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing. Proc Natl Acad Sci U S A. 2019,116(16):7738-7743. doi: 10.1073/pnas.1808645116
    Read this paper

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Figure 1. Western blot analysis of CD146 using anti-CD146 antibody (RP1050). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD146 antigen affinity purified polyclonal antibody (Catalog # RP1050) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD146 at approximately 120 kDa. The expected band size for CD146 is at 72 kDa.
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