CD146 antibody [P1H12]

GeneTex

CD146 antibody [P1H12]

9

Recognizes cultured microvascular and large-vessel endothelial cells (MVEC and HUVEC, respectively) and endothelial cells in tissues and in circulation.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen

Research Use Only

Monoclonal

P1H12

Unconjugated

MCAM

melanoma cell adhesion molecule

CD146; cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; Gicerin; HEMCAM; melanoma adhesion molecule; melanoma-associated antigen A32; melanoma-associated antigen MUC18; MelCAM; METCAM; MUC18; S-endo 1 endothelial-associated antigen

Mouse

IgG1

Cell surface glycoprotein MUC18

CD146 (also known as A32, MCAM, Mel-CAM, MUC18, and S-Endo-1) belongs to the immunoglobulin supergene family with five immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell-cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N-linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate-3-glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Overexpression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Human

-20°C or -80°C,2°C to 8°C

12352203