ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84795 CD147 antibody [5D8].
![IHC-P analysis of human colon adenocarcinoma tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_3125_IHC-P_w_23061420_493.webp "IHC-P analysis of human colon adenocarcinoma tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_3126_IHC-P_w_23061420_160.webp "IHC-P analysis of human ovary adenocarcinoma tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of human liver tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_3127_IHC-P_w_23061420_688.webp "IHC-P analysis of human liver tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of human pancreas tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_3128_IHC-P_w_23061420_445.webp "IHC-P analysis of human pancreas tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of human prostate tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_3129_IHC-P_w_23061420_724.webp "IHC-P analysis of human prostate tissue using GTX84795 CD147 antibody [5D8]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![WB analysis of HeLa cell lysate using GTX84795 CD147 antibody [5D8]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_4244_WB_w_23061420_238.webp "WB analysis of HeLa cell lysate using GTX84795 CD147 antibody [5D8]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with CD147 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84795 CD147 antibody [5D8]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_4627_WB_w_23061420_428.webp "WB analysis of HEK293T cells transfected with CD147 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84795 CD147 antibody [5D8]. Loading : 5 ug per lane")
![FACS analysis of HeLa cells using GTX84795 CD147 antibody [5D8]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_549_FACS_w_23061420_263.webp "FACS analysis of HeLa cells using GTX84795 CD147 antibody [5D8]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of Jurkat cells using GTX84795 CD147 antibody [5D8]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84795/GTX84795_550_FACS_w_23061420_127.webp "FACS analysis of Jurkat cells using GTX84795 CD147 antibody [5D8]. Red : Primary antibody Blue : Negative control antibody")
ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84795 CD147 antibody [5D8].
CD147 antibody [5D8]
GTX84795
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBSG
Overview
- SupplierGeneTex
- Product NameCD147 antibody [5D8] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5D8
- Concentration0.88 mg/ml
- ConjugateUnconjugated
- Gene ID682
- Target nameBSG
- Target descriptionbasigin (Ok blood group)
- Target synonyms5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, TCSF, basigin, OK blood group antigen, collagenase stimulatory factor, extracellular matrix metalloproteinase inducer, hepatoma-associated antigen, leukocyte activation antigen M6, tumor cell-derived collagenase stimulatory factor
- HostMouse
- IsotypeIgG2b
- Protein IDP35613
- Protein NameBasigin
- Scientific DescriptionPlays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- CD147 regulates the expression of MCT1 and lactate export in multiple myeloma cells. Walters DK et al., 2013 Oct 1, Cell CycleRead more
Datasheet
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