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CD147 Antibody(Rabbit)

HY-P80596
MedChem Express
TargetBsg
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CD147 Antibody(Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID12215
  • Target name
    Bsg
  • Target description
    basigin
  • Target synonyms
    CD147, EMMPRIN, HT-7, basigin, 5A11/Basigin, HT7 antigen, basic immunoglobulin superfamily, gp 42, membrane glycoprotein gp42, neurothelin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CD147 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD147.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD147/Bsg using anti-CD147/Bsg antibody (A00248-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147/Bsg antigen affinity purified polyclonal antibody (Catalog # A00248-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD147/Bsg at approximately 45 kDa. The expected band size for CD147/Bsg is at 45 kDa.
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