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WB analysis of mouse embryo tissue lysate using GTX37481 CD147 antibody.
WB analysis of mouse embryo tissue lysate using GTX37481 CD147 antibody.
WB analysis of mouse embryo tissue lysate using GTX37481 CD147 antibody.

CD147 antibody

GTX37481
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetBsg
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD147 antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID12215
  • Target name
    Bsg
  • Target description
    basigin
  • Target synonyms
    CD147, EMMPRIN, HT-7, basigin, 5A11/Basigin, HT7 antigen, basic immunoglobulin superfamily, gp 42, membrane glycoprotein gp42, neurothelin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18572
  • Protein Name
    Basigin
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CD147/Bsg using anti-CD147/Bsg antibody (A00248-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147/Bsg antigen affinity purified polyclonal antibody (Catalog # A00248-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD147/Bsg at approximately 45 kDa. The expected band size for CD147/Bsg is at 45 kDa.
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