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Cd160 Polyclonal Antibody

CAC11790
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCD160
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Overview

  • Supplier
    Biomatik
  • Product Name
    CD160 Antibody
  • Delivery Days Customer
    12
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC; Recommended dilution: IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11126
  • Target name
    CD160
  • Target description
    CD160 molecule
  • Target synonyms
    BY55, NK1, NK28, CD160 antigen, CD160-delta Ig, natural killer cell receptor BY55, natural killer cell receptor, immunoglobulin superfamily member
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95971
  • Protein Name
    CD160 antigen
  • Scientific Description
    The Cd160 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD160 using anti-CD160 antibody (A06168-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD160 antigen affinity purified polyclonal antibody (Catalog # A06168-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD160 at approximately 27KD. The expected band size for CD160 is at 20KD.
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