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Western Blot analysis of COLO205 cells using CD160 Polyclonal Antibody
Western Blot analysis of COLO205 cells using CD160 Polyclonal Antibody
Western Blot analysis of COLO205 cells using CD160 Polyclonal Antibody

CD160 Antibody

CSB-PA001426
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCD160
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD160 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11126
  • Target name
    CD160
  • Target description
    CD160 molecule
  • Target synonyms
    BY55; CD160 antigen; CD160-delta Ig; natural killer cell receptor BY55; natural killer cell receptor, immunoglobulin superfamily member; NK1; NK28
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95971
  • Protein Name
    CD160 antigen
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD160 using anti-CD160 antibody (A06168-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD160 antigen affinity purified polyclonal antibody (Catalog # A06168-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD160 at approximately 27KD. The expected band size for CD160 is at 20KD.
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