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ProductsBack/CD163 Antibody (OAEE00114)

CD163 Antibody (OAEE00114)

OAEE00114
Aviva Systems Biology
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetCD163
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CD163 Antibody (OAEE00114)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GHI/61
  • Concentration
    1 mg/ml
  • Estimated Purity
    >95%
  • Gene ID9332
  • Target name
    CD163
  • Target description
    CD163 molecule
  • Target synonyms
    hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; SCARI1; scavenger receptor cysteine-rich type 1 protein M130
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD163 using anti-CD163 antibody (A00812-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (Catalog # A00812-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD163 at approximately 150 kDa. The expected band size for CD163 is at 128 kDa.
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