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CD19 Antibody (aa422-433)

LS-C186459
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCD19
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD19 Antibody (aa422-433)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kD according to NP_NP_001761.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3 ug/ml.. Peptide-ELISA (1:32000), WB (1 - 3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kD according to NP_NP_001761.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID930
  • Target name
    CD19
  • Target description
    CD19 molecule
  • Target synonyms
    B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, T-cell surface antigen Leu-12, differentiation antigen CD19
  • Host
    Goat
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
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