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ProductsBack/CD19 antibody [C1C3]
CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
CD19 antibody [C1C3] detects CD19 protein at cell membrane by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD19 stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).

CD19 antibody [C1C3]

GTX101512
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCD19
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD19 antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.21 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID930
  • Target name
    CD19
  • Target description
    CD19 molecule
  • Target synonyms
    B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, T-cell surface antigen Leu-12, differentiation antigen CD19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15391
  • Protein Name
    B-lymphocyte antigen CD19
  • Scientific Description
    Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yasuda T, Saito Y, Ono C, et al. Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells. Sci Rep. 2021,11(1):5524. doi: 10.1038/s41598-021-84786-6
    Read this paper
  • Kurimoto C, Inaba H, Ariyasu H, et al. Predictive and sensitive biomarkers for thyroid dysfunctions during treatment with immune-checkpoint inhibitors. Cancer Sci. 2020,111(5):1468-1477. doi: 10.1111/cas.14363
    Read this paper
  • Bottero V, Sadagopan S, Johnson KE, et al. Kaposi's sarcoma-associated herpesvirus-positive primary effusion lymphoma tumor formation in NOD/SCID mice is inhibited by neomycin and neamine blocking angiogenin's nuclear translocation. J Virol. 2013,87(21):11806-20. doi: 10.1128/JVI.01920-13
    Read this paper

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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
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