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ProductsBack/Cd19 Rat Monoclonal Antibody [Clone ID: 6D5]
Stainin of Mouse spleen cells wit Rat Anti Mouse CD19 (SM018P).
Stainin of Mouse spleen cells wit Rat Anti Mouse CD19 (SM018P).
Stainin of Mouse spleen cells wit Rat Anti Mouse CD19 (SM018P).

Cd19 Rat Monoclonal Antibody [Clone ID: 6D5]

SM018PT
OriGene
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCd19
Price on request
25 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Cd19 Rat Monoclonal Antibody [Clone ID: 6D5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6D5
  • Gene ID12478
  • Target name
    Cd19
  • Target description
    CD19 antigen
  • Target synonyms
    B-lymphocyte antigen CD19, differentiation antigen CD19
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP25918
  • Protein Name
    B-lymphocyte antigen CD19
  • Scientific Description
    Cd19 rat monoclonal antibody, clone 6D5, Purified
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: human K562 whole cell lysates, Lane 6: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
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