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Western Blot analysis of K562 cells using CD1D Polyclonal Antibody
Western Blot analysis of K562 cells using CD1D Polyclonal Antibody
Western Blot analysis of K562 cells using CD1D Polyclonal Antibody

CD1D Antibody

CSB-PA005192
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCD1D
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD1D Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID912
  • Target name
    CD1D
  • Target description
    CD1d molecule
  • Target synonyms
    antigen-presenting glycoprotein CD1d; CD1A; CD1D antigen, d polypeptide; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D; R3; R3G1; T-cell surface glycoprotein CD1d; thymocyte antigen CD1D
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15813
  • Protein Name
    Antigen-presenting glycoprotein CD1d
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD1D using anti-CD1D antibody (A00323). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1D antigen affinity purified polyclonal antibody (Catalog # A00323) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1D at approximately 55KD. The expected band size for CD1D is at 38KD.
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