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mAb 7D6 staining on FDC clusters. Slides were incubated with mAb 7D6 and subsequently with a sheep anti-mouse IgG1. The binding of antibody was revealed using APAAP method and developed by Fast red substrate.
mAb 7D6 staining on FDC clusters. Slides were incubated with mAb 7D6 and subsequently with a sheep anti-mouse IgG1. The binding of antibody was revealed using APAAP method and developed by Fast red substrate.
mAb 7D6 staining on FDC clusters. Slides were incubated with mAb 7D6 and subsequently with a sheep anti-mouse IgG1. The binding of antibody was revealed using APAAP method and developed by Fast red substrate.

CD21 (CR2) (CD21L) Mouse Monoclonal Antibody [Clone ID: 7D6]

DDX0120P-100
OriGene
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetCR2
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CD21 (CR2) (CD21L) Mouse Monoclonal Antibody [Clone ID: 7D6]
  • Delivery Days Customer
    14
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7D6
  • Gene ID1380
  • Target name
    CR2
  • Target description
    complement C3d receptor 2
  • Target synonyms
    C3DR; CD21; complement component (3d/Epstein Barr virus) receptor 2; complement component 3d receptor 2; complement receptor type 2; CR; CVID7; EBV receptor; SLEB9
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20023
  • Protein Name
    Complement receptor type 2
  • Scientific Description
    CD21 (CR2) (CD21L) mouse monoclonal antibody, clone 7D6
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD21/CR2 using anti-CD21/CR2 antibody (A01632-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse Raw264.7 whole cell lysates, Lane 2: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD21/CR2 antigen affinity purified polyclonal antibody (Catalog # A01632-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD21/CR2 at approximately 100,150 kDa. The expected band size for CD21/CR2 is at 113 kDa.
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