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CD225 / IFITM1 Antibody (N-Terminus)

LS-C368733
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetIFITM1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD225 / IFITM1 Antibody (N-Terminus)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8519
  • Target name
    IFITM1
  • Target description
    interferon induced transmembrane protein 1
  • Target synonyms
    9-27; CD225; dispanin subfamily A member 2a; DSPA2a; IFI17; interferon-induced protein 17; interferon-induced transmembrane protein 1; interferon-inducible protein 9-27; LEU13; leu-13 antigen
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IFITM1 using anti-IFITM1 antibody (A02633-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFITM1 antigen affinity purified polyclonal antibody (Catalog # A02633-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFITM1 at approximately 17KD. The expected band size for IFITM1 is at 17KD.
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