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ProductsBack/IFITM1 Antibody
Western Blot analysis of MCF7, PC12 cells using CD225 Polyclonal Antibody.
Western Blot analysis of MCF7, PC12 cells using CD225 Polyclonal Antibody.
Western Blot analysis of MCF7, PC12 cells using CD225 Polyclonal Antibody.

IFITM1 Antibody

CSB-PA005866
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetIFITM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    IFITM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8519
  • Target name
    IFITM1
  • Target description
    interferon induced transmembrane protein 1
  • Target synonyms
    9-27; CD225; dispanin subfamily A member 2a; DSPA2a; IFI17; interferon-induced protein 17; interferon-induced transmembrane protein 1; interferon-inducible protein 9-27; LEU13; leu-13 antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13164
  • Protein Name
    Interferon-induced transmembrane protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IFITM1 using anti-IFITM1 antibody (A02633-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFITM1 antigen affinity purified polyclonal antibody (Catalog # A02633-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFITM1 at approximately 17KD. The expected band size for IFITM1 is at 17KD.
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