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CD23 Antibody (YA1296, Rabbit)

HY-P81551
MedChem Express
TargetFCER2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CD23 Antibody (YA1296, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2208
  • Target name
    FCER2
  • Target description
    Fc epsilon receptor II
  • Target synonyms
    BLAST-2, CD23, CD23A, CLEC4J, FCE2, FCErII, IGEBF, low affinity immunoglobulin epsilon Fc receptor, C-type lectin domain family 4, member J, CD23 antigen, Fc fragment of IgE receptor II, Fc fragment of IgE, low affinity II, receptor for (CD23), FcepsilonRII, fc-epsilon-RII, immunoglobulin E-binding factor, immunoglobulin epsilon-chain, lymphocyte IgE receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CD23 Antibody (YA1296) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1296), targeting CD23. CD23 Antibody (YA1296) can be used for IHC-P, ICC/IF, FC experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD23 using anti-CD23 antibody (A04237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD23 antigen affinity purified polyclonal antibody (Catalog # A04237) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD23 at approximately 36KD. The expected band size for CD23 is at 36KD.
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