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Anti-CD252/Tnfsf4 Antibody

ORB402502
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetTnfsf4
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Overview

  • Supplier
    Biorbyt
  • Product Name
    CD252/Tnfsf4 Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    Western blot, 0.1-0.5microg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1microg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID22164
  • Target name
    Tnfsf4
  • Target description
    tumor necrosis factor (ligand) superfamily, member 4
  • Target synonyms
    Ath; Ath-; Ath1; Ath-1; atherosclerosis 1; CD134; CD134L; gp3; gp34; OX4; OX40 ligand; Ox40l; OX-40L; tax-transcriptionally activated glycoprotein 1 ligand; Tnlg2b; tumor necrosis factor ligand 2b; tumor necrosis factor ligand superfamily member 4; Txgp; TXGP1; Txgp1l
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43488
  • Protein Name
    Tumor necrosis factor ligand superfamily member 4
  • Scientific Description
    Anti-CD252/Tnfsf4 Antibody. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD252 using anti-CD252 antibody (A02554-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CD252 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD252 antigen affinity purified polyclonal antibody (Catalog # A02554-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD252 at approximately 21-25KD. The expected band size for CD252 is at 18KD.
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