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ProductsBack/CD3 antibody [Hit3a] (FITC)
FACS analysis of human peripheral blood lymphocytes using GTX01459-06 CD3 antibody [Hit3a] (FITC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.5 microg (5 microl)
FACS analysis of human peripheral blood lymphocytes using GTX01459-06 CD3 antibody [Hit3a] (FITC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.5 microg (5 microl)
FACS analysis of human peripheral blood lymphocytes using GTX01459-06 CD3 antibody [Hit3a] (FITC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.5 microg (5 microl)

CD3 antibody [Hit3a] (FITC)

GTX01459-06
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
TargetCD3D
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD3 antibody [Hit3a] (FITC)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 0.5 microg (5 microl) for 105-108 cells in 100 microl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Hit3a
  • Concentration
    0.1 mg/ml
  • Conjugate
    FITC
  • Gene ID915
  • Target name
    CD3D
  • Target description
    CD3 delta subunit of T-cell receptor complex
  • Target synonyms
    CD3-DELTA, CD3DELTA, IMD19, T3D, T-cell surface glycoprotein CD3 delta chain, CD3 antigen, delta subunit, CD3 delta, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), OKT3, delta chain, T-cell receptor T3 delta chain
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP04234
  • Protein Name
    T-cell surface glycoprotein CD3 delta chain
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Lesourne R, Zvezdova E, Song KD, et al. Interchangeability of Themis1 and Themis2 in thymocyte development reveals two related proteins with conserved molecular function. J Immunol. 2012,189(3):1154-61. doi: 10.4049/jimmunol.1200123
    Read this paper
  • Knyazhitsky M, Moas E, Shaginov E, et al. Vav1 oncogenic mutation inhibits T cell receptor-induced calcium mobilization through inhibition of phospholipase Cγ1 activation. J Biol Chem. 2012,287(23):19725-35. doi: 10.1074/jbc.M111.309799
    Read this paper
  • Ge S, Hertel B, Emden SH, et al. Microparticle generation and leucocyte death in Shiga toxin-mediated HUS. Nephrol Dial Transplant. 2012,27(7):2768-75. doi: 10.1093/ndt/gfr748
    Read this paper
  • Soto PC, Stein LL, Hurtado-Ziola N, et al. Relative over-reactivity of human versus chimpanzee lymphocytes: implications for the human diseases associated with immune activation. J Immunol. 2010,184(8):4185-95. doi: 10.4049/jimmunol.0903420
    Read this paper
  • Westermann J, Bode U, Sahle A, et al. Naive, effector, and memory T lymphocytes efficiently scan dendritic cells in vivo: contact frequency in T cell zones of secondary lymphoid organs does not depend on LFA-1 expression and facilitates survival of effector T cells. J Immunol. 2005,174(5):2517-24.
    Read this paper
  • Mukouyama H, Janzen NK, Hernandez JM, et al. Generation of kidney cancer-specific antitumor immune responses using peripheral blood monocytes transduced with a recombinant adenovirus encoding carbonic anhydrase 9. Clin Cancer Res. 2004,10(4):1421-9.
    Read this paper

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Figure 1. Western blot analysis of CD3D using anti-CD3D antibody (A04405-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3D antigen affinity purified polyclonal antibody (Catalog # A04405-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3D at approximately 19 kDa. The expected band size for CD3D is at 19 kDa.
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