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ProductsBack/CD30 Antibody (Preservative Free)

CD30 Antibody (Preservative Free)

LS-C815466
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTNFRSF8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD30 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID943
  • Target name
    TNFRSF8
  • Target description
    TNF receptor superfamily member 8
  • Target synonyms
    CD30, D1S166E, Ki-1, tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, cytokine receptor CD30, lymphocyte activation antigen CD30
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CD30/TNFRSF8 using anti-CD30/TNFRSF8 antibody (A01225-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD30/TNFRSF8 antigen affinity purified polyclonal antibody (Catalog # A01225-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD30/TNFRSF8 at approximately 120KD. The expected band size for CD30/TNFRSF8 is at 64KD.
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