FACS analysis of BAEC cells using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
![FACS analysis of PBMC cells (green) using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX15842/GTX15842_1994_FACS_w_23060620_747.webp "FACS analysis of PBMC cells (green) using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde")
![ICC/IF analysis of THP-1 cells using GTX15842 CD31 antibody [HEC7]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15842/GTX15842_342_ICC-IF_w_23060620_952.webp "ICC/IF analysis of THP-1 cells using GTX15842 CD31 antibody [HEC7]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC")
FACS analysis of BAEC cells using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
CD31 antibody [HEC7]
GTX15842
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
ReactivityBovine, Human, Rat
TargetPECAM1
Overview
- SupplierGeneTex
- Product NameCD31 antibody [HEC7]
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:50-1:500. FACS: 3-5 microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHEC7
- Concentration1.06 mg/ml
- ConjugateUnconjugated
- Gene ID5175
- Target namePECAM1
- Target descriptionplatelet and endothelial cell adhesion molecule 1
- Target synonymsCD31; CD31 antigen; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1
- HostMouse
- IsotypeIgG2a
- Protein IDP16284
- Protein NamePlatelet endothelial cell adhesion molecule
- Scientific DescriptionThe protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]
- ReactivityBovine, Human, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203