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ProductsBack/CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: OTI13G9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC227758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC227758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC227758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A (1:500).

CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: OTI13G9]

CF500646
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetFCGR2A
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: OTI13G9]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI13G9
  • Gene ID2212
  • Target name
    FCGR2A
  • Target description
    Fc gamma receptor IIa
  • Target synonyms
    CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A1, FcGR, FcgammaRIIa, IGFR2, low affinity immunoglobulin gamma Fc region receptor II-a, Fc fragment of IgG receptor IIa, Fc fragment of IgG, low affinity IIa, receptor (CD32), Fc gamma receptor RIIa3, Immunoglobulin G Fc receptor II, fc-gamma-RIIa, fcRII-a, igG Fc receptor II-a
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP12318
  • Protein Name
    Low affinity immunoglobulin gamma Fc region receptor II-a
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FCGR2A mouse monoclonal antibody, clone OTI13G9 (formerly 13G9)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FCGR2A using anti-FCGR2A antibody (A01450-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates Lane 2: rat brain tissue lysates Lane 3: mouse Neuro-2a whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR2A antigen affinity purified polyclonal antibody (Catalog # A01450-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGR2A at approximately 40KD. The expected band size for FCGR2A is at 35KD.
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