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Western blot All lanes: CD33 antibody at 8microg/ml Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34, 26 kDa Observed band size: 40 kDa
Western blot All lanes: CD33 antibody at 8microg/ml Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34, 26 kDa Observed band size: 40 kDa
Western blot All lanes: CD33 antibody at 8microg/ml Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34, 26 kDa Observed band size: 40 kDa

CD33 Antibody

CSB-PA004925ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD33
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD33 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID945
  • Target name
    CD33
  • Target description
    CD33 molecule
  • Target synonyms
    CD33 antigen (gp67); CD33 molecule transcript; gp67; myeloid cell surface antigen CD33; p67; sialic acid-binding Ig-like lectin 3; SIGLEC3; SIGLEC-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20138
  • Protein Name
    Myeloid cell surface antigen CD33
  • Scientific Description
    Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD33 using anti-CD33 antibody (PB9932). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SKOV3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD33 antigen affinity purified polyclonal antibody (Catalog # PB9932) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD33 at approximately 45 kDa. The expected band size for CD33 is at 40 kDa.
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