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ProductsBack/CD34 Antibody (clone 581, APC)

CD34 Antibody (clone 581, APC)

LS-C812108
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetCD34
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD34 Antibody (clone 581, APC)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 microL of antibody per test (million cells in 100 microl staining volume or per 100 microl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    581
  • Conjugate
    APC (Allophycocyanin)
  • Estimated Purity
    ...
  • Gene ID947
  • Target name
    CD34
  • Target description
    CD34 molecule
  • Target synonyms
    CD34 antigen; hematopoietic progenitor cell antigen CD34
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD34 using anti-CD34 antibody (PB9053). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD.
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