ProductsBack/CD34 antibody [MEC14.7]

Staining of mouse H5V cells using CD34 antibody (GTX28158) and purified rat IgG2a isotype control.

CD34 antibody [MEC14.7]

GTX28158

ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Product group Antibodies

ReactivityHuman, Mouse, Rabbit

TargetCD34

Overview

Supplier
GeneTex
Product Name
CD34 antibody [MEC14.7]
Delivery Days Customer
9
Application Supplier Note
For ELISA: Use at an assay dependent dilution. For FACS: Use at a dilution of 1:500. For IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1:500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IHC-Fr: Use at a dilution of 1:500. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:500. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Applications
Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Certification
Research Use Only
Clonality
Monoclonal
Clone ID
MEC14.7
Concentration
1 mg/ml
Conjugate
Unconjugated
Gene ID947
Target name
CD34
Target description
CD34 molecule
Target synonyms
hematopoietic progenitor cell antigen CD34, CD34 antigen, CD34 sialomucin
Host
Rat
Isotype
IgG2a
Protein IDP28906
Protein Name
Hematopoietic progenitor cell antigen CD34
Scientific Description
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Reactivity
Human, Mouse, Rabbit
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203

References

Li K, Du Y, Cai Y, et al. Single-cell analysis reveals the chemotherapy-induced cellular reprogramming and novel therapeutic targets in relapsed/refractory acute myeloid leukemia. Leukemia. 2023,37(2):308-325. doi: 10.1038/s41375-022-01789-6
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Han C, Zhou ZX, Chen YR, et al. [Biological characteristics of sheep peripheral blood mesenchymal stem cell]. Beijing Da Xue Xue Bao Yi Xue Ban. 2022,54(6):1151-1157.
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Xiao W, Chen W, Wang Y, et al. Recombinant DTβ4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93(+)/CD34(+) cells for endothelialization. Sci Adv. 2022,8(28):eabn1958. doi: 10.1126/sciadv.abn1958
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Khorolskaya JI, Perepletchikova DA, Kachkin DV, et al. Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative Ophthalmology. Biomedicines. 2021,9(9). doi: 10.3390/biomedicines9091134
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Wang Z, Zhang B, et al. Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells. Stem Cells Int. 2021,2021:9947187. doi: 10.1155/2021/9947187
Read this paper
Kikuchi S, Morino T, Takagi R, et al. Development of a nasal mucosa-removal model for evaluating cell therapy. Regen Ther. 2021,16:32-41. doi: 10.1016/j.reth.2020.12.004
Read this paper
Xu H, Wang C, Liu C, et al. Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head. Stem Cells Transl Med. 2021,10(5):781-796. doi: 10.1002/sctm.20-0346
Read this paper
Chopra H, Han Y, Zhang C, et al. CD133(+)CD34(+) cells can give rise to EPCs: A comparative rabbit and human study. Blood Cells Mol Dis. 2021,86:102487. doi: 10.1016/j.bcmd.2020.102487
Read this paper
Zhan JW, Wang SQ, Feng MS, et al. Constant compression decreases vascular bud and VEGFA expression in a rabbit vertebral endplate ex vivo culture model. PLoS One. 2020,15(6):e0234747. doi: 10.1371/journal.pone.0234747
Read this paper
Nishi H, Ishii A, Ono I, et al. Biodegradable Flow Diverter for the Treatment of Intracranial Aneurysms: A Pilot Study Using a Rabbit Aneurysm Model. J Am Heart Assoc. 2019,8(20):e014074. doi: 10.1161/JAHA.119.014074
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CD34 antibody [MEC14.7]

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