More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/CD35 Monoclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD35 Monoclonal Antibody, Unconjugated (bsm-54759R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD35 Monoclonal Antibody, Unconjugated (bsm-54759R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD35 Monoclonal Antibody, Unconjugated (bsm-54759R) at 1:50 for 30 minutes at room temperature, DAB staining.

CD35 Monoclonal Antibody

BSM-54759R
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCR1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    CD35 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3H19
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1378
  • Target name
    CR1
  • Target description
    complement C3b/C4b receptor 1 (Knops blood group)
  • Target synonyms
    C3BR, C4BR, CD35, KN, complement receptor type 1, C3-binding protein, C3b/C4b receptor, CD35 antigen, Knops blood group antigen, complement component (3b/4b) receptor 1 (Knops blood group), complement receptor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17927
  • Protein Name
    Complement receptor type 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
Purified Anti-Human CD35 Antibody [E11]AGEL0257
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
ReactivityHuman
TargetCR1
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-CD35 [HB-8592]Ab00897-1.1
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, Other Application
ReactivityHuman, Monkey, Mouse, Primate
TargetCR1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CR1 Antibody144-61698
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman
TargetCR1
  • SizePrice
Product group Antibodies
Biosynth
CD35 antibody10R-2943
  • SizePrice
Figure 1. Western blot analysis of CD35 using anti-CD35 antibody (A01022-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human TF1 whole cell lysates, Lane 2: human TF1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD35 antigen affinity purified polyclonal antibody (Catalog # A01022-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD35 at approximately 250 kDa. The expected band size for CD35 is at 224 kDa.
Product group Antibodies
Boster Bio
Anti-CD35/CR1 Antibody Picoband(r)A01022-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCR1
  • SizePrice
Product group Antibodies
Biomatik
Cr1 Polyclonal AntibodyCAC10886
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCR1
  • SizePrice
Product group Antibodies
Bioss
CD35 Recombinant AntibodyBSM-60247R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCR1
  • SizePrice
Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA005932EA01HU at dilution of 1:100
Product group Antibodies
Cusabio
CR1 AntibodyCSB-PA005932EA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCR1
  • SizePrice
Product group Antibodies
GeneTex
CD35 antibody [E11] (FITC)GTX00483-06
ApplicationsFlow Cytometry
ReactivityHuman, Primate
TargetCR1
  • SizePrice
Immunohistochemistry analysis in human tonsil and skeletal muscle tissues using HPA042455 antibody. Corresponding CR1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-CR1 AntibodyHPA042455
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetCR1
  • SizePrice