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ProductsBack/CD36 Antibody (clone B325)

CD36 Antibody (clone B325)

LS-C170942
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
TargetCD36
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD36 Antibody (clone B325)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry
  • Applications Supplier
    Flo, IF (5 - 20 µg/ml), IHC (5 - 20 µg/ml) WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B325
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID948
  • Target name
    CD36
  • Target description
    CD36 molecule
  • Target synonyms
    BDPLT10; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); CHDS7; cluster determinant 36; FAT; fatty acid translocase; glycoprotein IIIb; GP3B; GP4; GPIIIB; GPIV; leukocyte differentiation antigen CD36; PAS IV; PAS-4 protein; PASIV; platelet glycoprotein 4; platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD36 using anti-CD36 antibody (A01189-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD36 antigen affinity purified polyclonal antibody (Catalog # A01189-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD36 at approximately 88 kDa. The expected band size for CD36 is at 53 kDa.
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