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Anti-CD36 [185.IG2], Human IgG1, kappa

AB04254-10.0
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
TargetCD36
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD36 [185.IG2], Human IgG1, kappa
  • Delivery Days Customer
    9
  • Application Supplier Note
    The original version of this antibody was used for immunoprecipitation on human blood platelets It was able to aggregate the same proteins as other CD36 specific antibodies (Miao et al., 2001; PMID:11238109). Further this antibody was used for immunohistochemistry on normal placentas and placentas infected with malaria (Hromatka et al., 2013; PMID:23575149), and on healthy and cancerous lymph nodes (Martens et al., 2006; PMID:16440291). This antibody was also used for immunofluorescence on healthy and cancerous lymph nodes to study lymph node sinuses as the primary site of tumour cell arrest upon regional lymph node metastasis (Martens et al., 2006; PMID:16440291). Finally this antibody was used to block the CD36 protein (Davies et al., 2001; PMID:11279149) (McIntyre et al., 2002; PMID:12502787) (Lopez-Vilchez et al., 2011; PMID:21641410).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    185.IG2
  • Gene ID948
  • Target name
    CD36
  • Target description
    CD36 molecule
  • Target synonyms
    BDPLT10; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 molecule (thrombospondin receptor); CHDS7; cluster determinant 36; FAT; fatty acid translocase; glycoprotein IIIb; GP3B; GP4; GPIIIB; GPIV; leukocyte differentiation antigen CD36; PAS IV; PAS-4 protein; PASIV; platelet glycoprotein 4; platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP16671
  • Protein Name
    Platelet glycoprotein 4
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD36 using anti-CD36 antibody (A01189-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD36 antigen affinity purified polyclonal antibody (Catalog # A01189-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD36 at approximately 88 kDa. The expected band size for CD36 is at 53 kDa.
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