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CD39 Antibody (clone A1, PE, Cy7)

LS-C812059
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetENTPD1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD39 Antibody (clone A1, PE, Cy7)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 microL of antibody per test (million cells in 100 microl staining volume or per 100 microl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.. Flo Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µl staining volume or per 100 µl of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A1
  • Conjugate
    Cy7, RPE
  • Estimated Purity
    ...
  • Gene ID953
  • Target name
    ENTPD1
  • Target description
    ectonucleoside triphosphate diphosphohydrolase 1
  • Target synonyms
    ATPDase; CD39; CD39 antigen; ecto-apyrase; ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; lymphoid cell activation antigen; NTPDase-1; SPG64
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD39/ENTPD1 using anti-CD39/ENTPD1 antibody (A03196-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD39/ENTPD1 antigen affinity purified polyclonal antibody (Catalog # A03196-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD39/ENTPD1 at approximately 80 kDa. The expected band size for CD39/ENTPD1 is at 80 kDa.
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