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Western Blot Positive WB detected in: MCF-7 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Rat stomach tissue All lanes: CD3G antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Rat stomach tissue All lanes: CD3G antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Rat stomach tissue All lanes: CD3G antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa

CD3G Antibody

CSB-PA004933LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCD3G
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD3G Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID917
  • Target name
    CD3G
  • Target description
    CD3 gamma subunit of T-cell receptor complex
  • Target synonyms
    CD3-GAMMA, CD3GAMMA, IMD17, T3G, T-cell surface glycoprotein CD3 gamma chain, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), T-cell antigen receptor complex, gamma subunit of T3, T-cell receptor T3 gamma chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09693
  • Protein Name
    T-cell surface glycoprotein CD3 gamma chain
  • Scientific Description
    The CD3 complex mediates signal transduction.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CD3 Gamma/CD3G using anti-CD3 Gamma/CD3G antibody (A04853-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOMLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3 Gamma/CD3G antigen affinity purified polyclonal antibody (Catalog # A04853-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3 Gamma/CD3G at approximately 20-25 kDa. The expected band size for CD3 Gamma/CD3G is at 20-25 kDa.
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