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ProductsBack/CD4 antibody [W3/25]
IHC-Fr stainng of rat lymph node with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 (GTX76318) in green. Merged image is on the right. High power
IHC-Fr stainng of rat lymph node with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 (GTX76318) in green. Merged image is on the right. High power
IHC-Fr stainng of rat lymph node with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 (GTX76318) in green. Merged image is on the right. High power

CD4 antibody [W3/25]

GTX76318
GeneTex
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityRat
TargetCd4
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD4 antibody [W3/25]
  • Delivery Days Customer
    9
  • Application Supplier Note
    FCM: 1/100-1/200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    W3/25
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID24932
  • Target name
    Cd4
  • Target description
    Cd4 molecule
  • Target synonyms
    W3/25, p55, T-cell surface glycoprotein CD4, CD4 antigen, T-cell surface antigen T4/Leu-3, W3/25 antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05540
  • Protein Name
    T-cell surface glycoprotein CD4
  • Scientific Description
    MHC class II binding protein that may be a candidate gene for collagen-induced arthritis; human CD4 is a receptor required for HIV infection [RGD, Feb 2006]
  • Reactivity
    Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD4 using anti-CD4 antibody (A00344-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 antigen affinity purified polyclonal antibody (Catalog # A00344-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4 at approximately 52KD. The expected band size for CD4 is at 52KD.
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IHC-Fr staining of rat lymph node using Mouse anti Rat CD4 antibody (GTX75110), red in A and Mouse anti Rat CD8, green in B. C is merged image with nuclear counterstaining with DAPI in blue. Low power
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