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ProductsBack/PerCP Anti-Rat CD4 (domain 1) Antibody [OX-38]

PerCP Anti-Rat CD4 (domain 1) Antibody [OX-38]

AGEL2208
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityRat
TargetCd4
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PerCP Anti-Rat CD4 (domain 1) Antibody [OX-38]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-38
  • Conjugate
    PerCP
  • Gene ID24932
  • Target name
    Cd4
  • Target description
    Cd4 molecule
  • Target synonyms
    W3/25, p55, T-cell surface glycoprotein CD4, CD4 antigen, T-cell surface antigen T4/Leu-3, W3/25 antigen
  • Host
    Mouse
  • Protein IDP05540
  • Protein Name
    T-cell surface glycoprotein CD4
  • Scientific Description
    PerCP Anti-Rat CD4 (domain 1) Antibody [OX-38] is a high-performance monoclonal that specifically targets Rat CD4 (domain 1), delivering exceptional specificity and reliability for your research applications. Rigorously validated for FCM, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100 Tests format to meet your experimental needs. For optimal performance, store at 4°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Mouse
  • Reactivity
    Rat
  • Reactivity Supplier
    Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CD4 using anti-CD4 antibody (A00344-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 antigen affinity purified polyclonal antibody (Catalog # A00344-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4 at approximately 52KD. The expected band size for CD4 is at 52KD.
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