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ProductsBack/CD40 Mouse Monoclonal Antibody [Clone ID: G28-5]
IHC staining of formalin-fixed paraffin-embedded human cervical tissue with mAb G28.5 Altenburg A et al, J.Immunol, 1999. a: normal cervical epithelium b: cervical intraepithelial neoplasia c: squamous cell carcinoma
IHC staining of formalin-fixed paraffin-embedded human cervical tissue with mAb G28.5 Altenburg A et al, J.Immunol, 1999. a: normal cervical epithelium b: cervical intraepithelial neoplasia c: squamous cell carcinoma
IHC staining of formalin-fixed paraffin-embedded human cervical tissue with mAb G28.5 Altenburg A et al, J.Immunol, 1999. a: normal cervical epithelium b: cervical intraepithelial neoplasia c: squamous cell carcinoma

CD40 Mouse Monoclonal Antibody [Clone ID: G28-5]

DDX0130P-100
OriGene
ApplicationsFunctional Assay, Flow Cytometry, ImmunoHistoChemistry
Product group Antibodies
TargetCD40
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CD40 Mouse Monoclonal Antibody [Clone ID: G28-5]
  • Delivery Days Customer
    14
  • Applications
    Functional Assay, Flow Cytometry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    G28-5
  • Gene ID958
  • Target name
    CD40
  • Target description
    CD40 molecule
  • Target synonyms
    Bp50, CDW40, TNFRSF5, p50, tumor necrosis factor receptor superfamily member 5, B cell surface antigen CD40, B cell-associated molecule, CD40 molecule, TNF receptor superfamily member 5, CD40L receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP25942
  • Protein Name
    Tumor necrosis factor receptor superfamily member 5
  • Scientific Description
    CD40 mouse monoclonal antibody, clone G28-5
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10051). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40/TNFRSF5 antigen affinity purified polyclonal antibody (Catalog # PB10051) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40/TNFRSF5 at approximately 43 kDa. The expected band size for CD40/TNFRSF5 is at 30 kDa.
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