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ProductsBack/CD40 Mouse Monoclonal Antibody [Clone ID: MAB89]
IHC staining on Bouin paraffin lymph node section. Human CD40 stained with mAb 89.
IHC staining on Bouin paraffin lymph node section. Human CD40 stained with mAb 89.
IHC staining on Bouin paraffin lymph node section. Human CD40 stained with mAb 89.

CD40 Mouse Monoclonal Antibody [Clone ID: MAB89]

DDX0131P-100
OriGene
ApplicationsFunctional Assay, Flow Cytometry, ImmunoHistoChemistry
Product group Antibodies
TargetCD40
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CD40 Mouse Monoclonal Antibody [Clone ID: MAB89]
  • Delivery Days Customer
    14
  • Applications
    Functional Assay, Flow Cytometry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MAB89
  • Gene ID958
  • Target name
    CD40
  • Target description
    CD40 molecule
  • Target synonyms
    Bp50, CDW40, TNFRSF5, p50, tumor necrosis factor receptor superfamily member 5, B cell surface antigen CD40, B cell-associated molecule, CD40 molecule, TNF receptor superfamily member 5, CD40L receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP25942
  • Protein Name
    Tumor necrosis factor receptor superfamily member 5
  • Scientific Description
    CD40 mouse monoclonal antibody, clone MAB89
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10051). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40/TNFRSF5 antigen affinity purified polyclonal antibody (Catalog # PB10051) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40/TNFRSF5 at approximately 43 kDa. The expected band size for CD40/TNFRSF5 is at 30 kDa.
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