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FACS analysis of human PBMCs (with PMA and Ionomycin stimulation for 5 hrs) using GTX01484-07 CD40L / CD154 antibody [5C8] (APC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 microg (5 microl)

CD40L / CD154 antibody [5C8] (APC)

GTX01484-07

ApplicationsFlow Cytometry

Product group Antibodies

ReactivityHuman, Monkey, Primate

TargetCD40LG

Overview

Supplier
GeneTex
Product Name
CD40L / CD154 antibody [5C8] (APC)
Delivery Days Customer
9
Application Supplier Note
FACS: 0.25 microg (5 microl) for 105-108 cells in 100 microl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
Flow Cytometry
Certification
Research Use Only
Clonality
Monoclonal
Clone ID
5C8
Concentration
0.05 mg/ml
Conjugate
APC (Allophycocyanin)
Gene ID959
Target name
CD40LG
Target description
CD40 ligand
Target synonyms
CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand, CD40 antigen ligand, CD40-L, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, tumor necrosis factor (ligand) superfamily member 5
Host
Mouse
Isotype
IgG2a
Protein IDP29965
Protein Name
CD40 ligand
Scientific Description
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
Reactivity
Human, Monkey, Primate
Storage Instruction
2°C to 8°C
UNSPSC
12352203

References

Koehler FC, Cornely OA, Wisplinghoff H, et al. Candida-Reactive T Cells for the Diagnosis of Invasive Candida Infection-A Prospective Pilot Study. Front Microbiol. 2018,9:1381. doi: 10.3389/fmicb.2018.01381
Read this paper
Geißler K, Markwart R, Requardt RP, et al. Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis. PLoS One. 2017,12(9):e0183214. doi: 10.1371/journal.pone.0183214
Read this paper
Jürchott K, Schulz AR, Bozzetti C, et al. Highly Predictive Model for a Protective Immune Response to the A(H1N1)pdm2009 Influenza Strain after Seasonal Vaccination. PLoS One. 2016,11(3):e0150812. doi: 10.1371/journal.pone.0150812
Read this paper
Stervbo U, Bozzetti C, Baron U, et al. Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets. Age (Dordr). 2015,37(5):93. doi: 10.1007/s11357-015-9829-2
Read this paper
Schmied S, Gostick E, Price DA, et al. Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4(+) and CD8(+) memory formation. Immunology. 2015,145(4):558-69. doi: 10.1111/imm.12472
Read this paper

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CD40L / CD154 antibody [5C8] (APC)

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Overview

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