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ProductsBack/Anti-Human CD154 (CD40L) Antibody, RayBright(r) Red 647

Anti-Human CD154 (CD40L) Antibody, RayBright(r) Red 647

136-24036
RayBiotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetCD40LG
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Human CD154 (CD40L) Antibody, RayBright(r) Red 647
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5c8
  • Concentration
    5 ul/test
  • Conjugate
    Other Conjugate
  • Gene ID959
  • Target name
    CD40LG
  • Target description
    CD40 ligand
  • Target synonyms
    CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand, CD40 antigen ligand, CD40-L, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, tumor necrosis factor (ligand) superfamily member 5
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP29965
  • Protein Name
    CD40 ligand
  • Scientific Description
    Human CD154 (CD40L) Antibody [clone: 5c8], monoclonal mouse IgG2a kappa. Validated for flow cytometry.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (A01114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody (Catalog # A01114-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40L at approximately 19KD. The expected band size for CD40L is at 29KD.
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