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ProductsBack/CD44 (21-145) Mouse Monoclonal Antibody [Clone ID: 5C10]
The Cell lysates of U937 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD44 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The Cell lysates of U937 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD44 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The Cell lysates of U937 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD44 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

CD44 (21-145) Mouse Monoclonal Antibody [Clone ID: 5C10]

AM03178PU-N
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD44
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    CD44 (21-145) Mouse Monoclonal Antibody [Clone ID: 5C10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C10
  • Gene ID960
  • Target name
    CD44
  • Target description
    CD44 molecule (IN blood group)
  • Target synonyms
    CDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, CD44 molecule (Indian blood group), GP90 lymphocyte homing/adhesion receptor, Hermes antigen, In(Lu) related-p80, Indian blood group antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, epican, extracellular matrix receptor III, hematopoietic cell E- and L-selectin ligand, heparan sulfate proteoglycan, homing cell adhesion molecule, homing function and Indian blood group system, hyaluronate receptor, phagocyte glycoprotein 1, phagocytic glycoprotein 1, soluble CD44
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP16070
  • Protein Name
    CD44 antigen
  • Scientific Description
    CD44 (21-145) mouse monoclonal antibody, clone 5C10, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CD44 using anti-CD44 antibody (A00052-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (Catalog # A00052-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44 at approximately 85 kDa. The expected band size for CD44 is at 82 kDa.
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