Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637231/GTX637231_T-44746_20220902_WB_competitor_watermark_22090701_780.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.")
![CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637231/GTX637231_T-44746_20221202_ICC_IF_22120719_700.webp "CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637231/GTX637231_44802_20221223_WB_C_22122722_808.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog lung. CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637231/GTX637231_44802_20230203_IHC-P_Dog_23020621_368.webp "CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog lung. CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat mammary gland. CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637231/GTX637231_44802_20230203_IHC-P_Cat_23020621_398.webp "CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat mammary gland. CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637231/GTX637231_44802_20230324_IHC-P_23032819_228.webp "CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637231/GTX637231_T-44746_20230707_WB_23071223_379.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637231/GTX637231_44802_20230707_WB_treatment_Tunicamycin_23071223_683.webp "Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![CD44 antibody [HL1650] (GTX637231) detects CD44 protein by flow cytometry analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at 4oC for 15 min and washed with staining buffer. The cells were following stained with CD44 antibody [HL1650] (GTX637231) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4oC for 1 hour.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637231/GTX637231_45278_20250919_FCM_25100122_940.webp "CD44 antibody [HL1650] (GTX637231) detects CD44 protein by flow cytometry analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at 4oC for 15 min and washed with staining buffer. The cells were following stained with CD44 antibody [HL1650] (GTX637231) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4oC for 1 hour.")
Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CD44 antibody [HL1650]
GTX637231
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Feline, Human
TargetCD44
Overview
- SupplierGeneTex
- Product NameCD44 antibody [HL1650]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1650
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID960
- Target nameCD44
- Target descriptionCD44 molecule (IN blood group)
- Target synonymsCDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen, CD44 molecule (Indian blood group), GP90 lymphocyte homing/adhesion receptor, Hermes antigen, In(Lu) related-p80, Indian blood group antigen, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, epican, extracellular matrix receptor III, hematopoietic cell E- and L-selectin ligand, heparan sulfate proteoglycan, homing cell adhesion molecule, homing function and Indian blood group system, hyaluronate receptor, phagocyte glycoprotein 1, phagocytic glycoprotein 1, soluble CD44
- HostRabbit
- IsotypeIgG
- Protein IDP16070
- Protein NameCD44 antigen
- Scientific DescriptionThe protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
- ReactivityCanine, Feline, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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