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ProductsBack/Cd46 Polyclonal Antibody

Cd46 Polyclonal Antibody

CAC07492
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCd46
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Overview

  • Supplier
    Biomatik
  • Product Name
    Cd46 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID17221
  • Target name
    Cd46
  • Target description
    CD46 antigen, complement regulatory protein
  • Target synonyms
    Mcp, membrane cofactor protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO88174
  • Protein Name
    Membrane cofactor protein
  • Scientific Description
    The Cd46 Polyclonal Antibody (Species: Mouse) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD46 using anti-CD46 antibody (PB9848). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates, Lane 2: NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD46 antigen affinity purified polyclonal antibody (Catalog # PB9848) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD46 at approximately 41 kDa, 70 kDa. The expected band size for CD46 is at 41 kDa.
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