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Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: Cd46 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 34 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: Cd46 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 34 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: Cd46 antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 34 kDa Observed band size: 41 kDa

Cd46 Antibody

CSB-PA004939LA01MO
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCd46
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Overview

  • Supplier
    Cusabio
  • Product Name
    Cd46 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID17221
  • Target name
    Cd46
  • Target description
    CD46 antigen, complement regulatory protein
  • Target synonyms
    Mc; Mcp; membrane cofactor protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO88174
  • Protein Name
    Membrane cofactor protein
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD46 using anti-CD46 antibody (PB9848). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates, Lane 2: NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD46 antigen affinity purified polyclonal antibody (Catalog # PB9848) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD46 at approximately 41 kDa, 70 kDa. The expected band size for CD46 is at 41 kDa.
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